2D Diagonal Redox SDS-PAGE of Proteins

2D Diagonal Redox SDS-PAGE of Proteins

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2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).Both dimensions consist of read more a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent.Intermolecular disulfide bridges increase the apparent molecular weight of read more a protein in the first dimension, whereas intramolecular bridges decrease the apparent weight of the protein.